Synergistic interaction of two antimalarial drugs, artemisinin and concanamycin A

Artemisinin is a powerful drug that has been combined with other antimalarial drugs to combat malaria and it crucial recent achievements in reducing cases. However, the emergence of Plasmodium falciparum resistance against artemisinin become serious problem treatment. Our previous studies reported alkalinised digestive vacuole P. similarly concanamycin A. Concanamycin A specific inhibitor V-type H+-ATPase, proton pump located on membrane vacuole. study also showed low concentration required kill 50% parasites. Therefore, this aimed determine interaction by using isobologram analysis effects parasite growth. The activity (IC50) was evaluated malarial SBYR Green I fluorescence-based (MSF) assay prior analysis. Based their IC50 values, six different combination solutions were assigned used 13 ± 2.52 nM 7 1.15 nM, respectively. found be synergistic, indicating these could parasites more effectively. This suggests can new candidate artemisinin-based therapies.